Abstract<\/strong><\/p>\n\n\n\n Photothermal therapy (PTT) is a promising approach for cancer targeting therapy. However, the temperature-dependent killing of tumor cells in PTT remains unclear. In this study, we report necroptosis plays a role in the anti-tumor effects observed in gold nanorod (GNR)-mediated PTT in melanoma. We first synthesized gold nanorods with a targeting adaptor FA (GNRs-FA), which achieved high efficacy of targeted delivery to melanoma cells. We further demonstrated PTT, precipitated by GNRs-FA under the induction of near-infrared laser, was temperature-dependent. Furthermore, the photothermal killing of melanoma cells showed different patterns of cell death depending on varying temperature in PTT.<\/p>\n\n\n\n In a lower temperature at 43\u2009\u00b0C, the percentages of apoptosis, necroptosis and necrosis of tumor cells were 10.2%, 18.3%, and 17.6%, respectively, suggesting the cell killing is ineffective at lower temperatures. When the temperature increased to 49\u2009\u00b0C, the cell death pattern switched to necrosis dominant (52.8%). Interestingly, when the PTT achieved a moderate temperature of 46\u2009\u00b0C, necroptosis was significantly increased (35.1%). Additionally, GNRs-FA\/PPT-mediated necroptosis was regulated by RIPK1 pathway.<\/p>\n\n\n\n Taken together, this study is the first to demonstrate that temperature-dependent necroptosis is an important mechanism of inducing melanoma cell<\/a> death in GNR-mediated PTT in addition to apoptosis and necrosis.<\/p>\n\n\n\n Author Contributions<\/strong> View Full Text<\/a> Here<\/a><\/p>","protected":false},"excerpt":{"rendered":" Temperature-dependent cell death patterns induced by functionalized gold nanoparticle photothermal therapy in melanoma cells Abstract Photothermal therapy (PTT) is a promising approach for cancer targeting therapy. However, the temperature-dependent killing of tumor cells in PTT remains unclear. In this study, we report necroptosis plays a role in the anti-tumor effects observed in gold nanorod (GNR)-mediated […]<\/p>\n","protected":false},"author":8,"featured_media":237204,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"content-type":"","footnotes":""},"categories":[7,46],"tags":[140,56,58,20,57,141],"class_list":["post-236994","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general","category-support","tag-ptt","tag-rd","tag-research","tag-skin-cancer","tag-skin-disease","tag-therapy"],"_links":{"self":[{"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/posts\/236994","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/comments?post=236994"}],"version-history":[{"count":12,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/posts\/236994\/revisions"}],"predecessor-version":[{"id":241841,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/posts\/236994\/revisions\/241841"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/media\/237204"}],"wp:attachment":[{"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/media?parent=236994"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/categories?post=236994"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/getskinhelp.com\/ch\/wp-json\/wp\/v2\/tags?post=236994"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
Yujuan Zhang, Xuelin Zhan, Juan Xiong, Shanshan Peng, Wei Huang, Rakesh Joshi, Ying Cai, Yanling Liu, Rong Li, Keng Yuan, Nanjin Zhou & Weiping Min<\/p>\n\n\n\n